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Year 9 Biology revision questions

Year 9 is the awkward bridge year: still Key Stage 3 in school life, but already starting GCSE habits. This page gives free Year 9 Biology practice from GradeNinja's own bank - 58 auto-marked questions across 2 Year 9 topic sets.

What Year 9 revision should do

The aim is not to cram GCSE papers early. It is to make the first GCSE topics feel normal: short retrieval practice, clear corrections, and enough repetition that classwork sticks before Year 10 gets busy.

Use these as ten-minute checks after a lesson or before homework. If you miss one, read the explanation, then come back tomorrow and answer it cold.

How GradeNinja pitches it

Year 9 questions are pulled from late Key Stage 3 and foundation GCSE bridge material. They should feel harder than Year 8, but they are not meant to be full Year 11 exam questions.

Year 9 (Starting GCSE) - Practice Mix54 questions
Year 9 (Starting GCSE)26 questions

Best way to use this page

Answer first, then reveal. The useful bit is the retrieval attempt, not reading the answer. If a topic keeps going wrong, paste the homework or Google Classroom text into GradeNinja's Classroom import tool and turn it into a focused pack.

Year 9 Biology practice questions

Real questions from GradeNinja's own bank, with the answer and the explanation the app gives. Tap to reveal.

Question 1 · Year 9 (Starting GCSE) - Practice Mix
Enzymes are biological molecules that act as:
  1. Energy stores
  2. Structural materials
  3. Catalysts for chemical reactions
  4. Hormones
Show the answer

Catalysts for chemical reactions

Enzymes are biological catalysts — they speed up chemical reactions in living organisms without being used up themselves.

Question 2 · Year 9 (Starting GCSE) - Practice Mix
In genetics, what term describes an allele that is always expressed when present, even in a heterozygous individual?
  1. Recessive
  2. Co-dominant
  3. Dominant
  4. Polygenic
Show the answer

Dominant

A dominant allele is always expressed in the phenotype whenever it is present, whether the individual is homozygous (two copies) or heterozygous (one copy). It masks the effect of a recessive allele.

Question 3 · Year 9 (Starting GCSE) - Practice Mix
Which enzyme breaks down starch beginning in the mouth?
  1. Lipase
  2. Protease
  3. Amylase
  4. Pepsin
Show the answer

Amylase

Salivary amylase is produced in the salivary glands and begins the digestion of starch in the mouth, breaking it down into maltose. Amylase is also produced by the pancreas and continues starch digestion in the small intestine.

Question 4 · Year 9 (Starting GCSE) - Practice Mix
What is a community in ecology?
  1. All the organisms of one species in a habitat
  2. All the organisms of different species living together in a habitat
  3. The physical environment where organisms live
  4. A group of habitats forming a larger area
Show the answer

All the organisms of different species living together in a habitat

A community is all the organisms of different species living together in a habitat. A single species group is a population.

Question 5 · Year 9 (Starting GCSE) - Practice Mix
In a food chain, what term describes an organism that makes its own food through photosynthesis?
  1. Consumer
  2. Decomposer
  3. Producer
  4. Predator
Show the answer

Producer

Producers (usually green plants or algae) make their own food using sunlight via photosynthesis. They are always at the start of a food chain.

Question 6 · Year 9 (Starting GCSE) - Practice Mix
What does a pyramid of biomass show?
  1. The number of organisms at each trophic level
  2. The mass of living material at each trophic level
  3. The energy released by each organism
  4. The speed of nutrient cycling
Show the answer

The mass of living material at each trophic level

A pyramid of biomass shows the total mass of living material (dry mass) at each trophic level. It is nearly always pyramid-shaped because energy is lost at each stage — typically only about 10% of energy passes from one level to the next (the rest is lost as heat, waste, movement).

Question 7 · Year 9 (Starting GCSE) - Practice Mix
In a monohybrid cross between Aa and Aa, what percentage of offspring are homozygous?
  1. 25%
  2. 50%
  3. 75%
  4. 100%
Show the answer

25%

In a monohybrid cross of Aa x Aa, the offspring can be AA, Aa, or aa. The homozygous offspring (AA and aa) represent 25% of the total, specifically 1 out of 4 being AA and 1 out of 4 being aa. Remember the ratio of genotypes produced in this cross.

Question 8 · Year 9 (Starting GCSE)
Enzymes are biological catalysts. What happens to an enzyme above its optimum temperature?
  1. It denatures — the active site changes shape permanently
  2. It works faster
  3. It freezes
  4. It becomes more specific
Show the answer

It denatures — the active site changes shape permanently

Above the optimum temperature, the enzyme denatures. The bonds holding its 3D shape break, so the active site changes shape and the substrate can no longer fit. This is irreversible. Below optimum temperature, enzymes still work but more slowly.

Question 9 · Year 9 (Starting GCSE)
Why do villi in the small intestine help absorption?
  1. They reduce surface area
  2. They increase surface area and have a good blood supply
  3. They make enzymes in the stomach
  4. They stop diffusion happening
Show the answer

They increase surface area and have a good blood supply

Villi give a large surface area, thin walls and a good blood supply, so digested food molecules are absorbed quickly.

Question 10 · Year 9 (Starting GCSE) - Practice Mix
Which model best describes how an enzyme works with its substrate?
  1. Induced fit model
  2. Lock and key model
  3. Fluid mosaic model
  4. Sliding filament model
Show the answer

Lock and key model

The lock and key model describes how the substrate (the "key") fits precisely into the active site of the enzyme (the "lock"). Only the correctly shaped substrate fits, which is why enzymes are specific to one reaction. The induced fit model is a more advanced version but lock and key is the AQA GCSE model.

Question 11 · Year 9 (Starting GCSE)
Which cell structure controls which substances enter and leave the cell?
  1. Nucleus
  2. Cell membrane
  3. Ribosome
  4. Mitochondrion
Show the answer

Cell membrane

The cell membrane controls movement into and out of the cell. The nucleus controls cell activities and contains DNA.

Question 12 · Year 9 (Starting GCSE) - Practice Mix
What is an ecosystem?
  1. A group of organisms of the same species
  2. All the living organisms in an area and their non-living environment
  3. The study of habitats
  4. A food web in the ocean
Show the answer

All the living organisms in an area and their non-living environment

An ecosystem consists of all living organisms in a specific area, along with their non-living environment, such as soil, water, and climate. This concept is fundamental in ecology, as it shows the interactions between biotic and abiotic components. Remember, ecosystems are about relationships in nature.

Question 13 · Year 9 (Starting GCSE) - Practice Mix
Which products are formed during anaerobic respiration in yeast?
  1. Carbon dioxide and water
  2. Lactic acid only
  3. Ethanol and carbon dioxide
  4. Glucose and oxygen
Show the answer

Ethanol and carbon dioxide

Yeast carries out anaerobic respiration (fermentation) producing ethanol (alcohol) and carbon dioxide. This is exploited in bread-making (CO2 causes rising) and brewing (ethanol production).

Question 14 · Year 9 (Starting GCSE) - Practice Mix
What is the correct word equation for photosynthesis?
  1. Carbon dioxide + water → glucose + oxygen
  2. Glucose + oxygen → carbon dioxide + water
  3. Carbon dioxide + glucose → water + oxygen
  4. Water + oxygen → carbon dioxide + glucose
Show the answer

Carbon dioxide + water → glucose + oxygen

Photosynthesis: CO₂ + H₂O → C₆H₁₂O₆ + O₂. Plants absorb carbon dioxide from the air and water from the soil, then use light energy to make glucose and release oxygen as a by-product.

Question 15 · Year 9 (Starting GCSE) - Practice Mix
Salmonella food poisoning is caused by:
  1. A virus
  2. A bacterium ingested in contaminated food, such as undercooked poultry or eggs
  3. A fungus
  4. A protist
Show the answer

A bacterium ingested in contaminated food, such as undercooked poultry or eggs

Salmonella is a bacterium that can infect the gut, digestive system, and body of farm animals, spreading to humans through contaminated food.

Question 16 · Year 9 (Starting GCSE) - Practice Mix
DNA is a double helix made up of two strands linked by complementary base pairs. Which bases pair together?
  1. Adenine–Guanine and Cytosine–Thymine
  2. Adenine–Thymine and Cytosine–Guanine
  3. Adenine–Cytosine and Guanine–Thymine
  4. Adenine–Uracil and Guanine–Cytosine
Show the answer

Adenine–Thymine and Cytosine–Guanine

In DNA: adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). This complementary base pairing holds the two strands together.

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Common questions

Are these Year 9 Biology questions GCSE level?

They are bridge questions: harder than early Key Stage 3, but not pitched as full Year 11 GCSE exam papers. The point is to prepare the habits and core knowledge early.

Can I use Google Classroom homework with GradeNinja?

Yes. Use Classroom import in the app: paste the Google Classroom post, homework email or assignment brief and it turns the topics into practice questions and flashcards.

How often should a Year 9 revise Biology?

Short and often. Ten minutes after a lesson or a few times a week is more useful than one long session before a test.

Is GradeNinja free for Year 9?

Yes, signing up is free. GradeNinja has Year 9 bridge content plus the wider question bank across 28 subjects.

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