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GCSE biology revision questions

Free GCSE biology practice for Year 11, nine months out from the May/June 2027 exams. Every question below comes from GradeNinja's own bank - 2,406 Biology questions across 54 topics.

What to prioritise in Biology

Biology carries more pure recall than the other two sciences, which makes it the best possible fit for spaced retrieval practice. The content is not hard; there is simply a lot of it, and it fades fast if you meet each topic once.

The required practicals are worth targeting early. They come up reliably, they are finite, and most people leave them until they are panicking in April.

Exam board

Our Biology questions follow the AQA specification. If your school uses a different board most of the content still overlaps, but check your own spec.

How to use these

Answer before you open the explanation. The moment of not-quite-knowing is the part that does the work, and skipping to the answer skips the learning.

Anything you get wrong, come back to tomorrow rather than immediately. Getting it right straight after seeing the answer proves nothing.

The nine months ahead

Biology practice questions

Real questions from GradeNinja's own bank, with the answer and the explanation the app gives. Tap to reveal.

Question 1 · Required Practicals (AQA)
In a microscopy practical, a student observes cells. They can see the cell membrane and nucleus but cannot see mitochondria. The most likely reason is:
  1. The cells do not contain mitochondria
  2. Mitochondria are too small to be resolved by a light microscope
  3. The stain used does not bind to mitochondria
  4. Mitochondria are only found in plant cells
Show the answer

Mitochondria are too small to be resolved by a light microscope

Light microscopes have a resolution limit of about 200 nm (0.2 µm). Mitochondria are 0.5–10 µm in length — technically within the size range, but their internal membrane detail cannot be resolved by light microscopy. Electron microscopes (resolution ~0.1 nm) are needed to see mitochondrial cristae and other fine ultrastructure.

Question 2
Which structure controls what enters and leaves a cell?
  1. Cell wall
  2. Cytoplasm
  3. Cell membrane
  4. Nucleus
Show the answer

Cell membrane

The cell (plasma) membrane is selectively permeable and controls the passage of substances into and out of the cell.

Question 3 · Evolution & Genetics
Which of the following best describes how antibiotic resistance develops in bacterial populations?
  1. Random mutations create resistant bacteria before antibiotics are used; antibiotics select for them
  2. Antibiotics cause bacteria to mutate
  3. Bacteria learn to resist antibiotics over time
  4. Patients transfer resistance to bacteria
Show the answer

Random mutations create resistant bacteria before antibiotics are used; antibiotics select for them

Resistant bacteria arise by chance mutation before antibiotic exposure. When antibiotics are used, sensitive bacteria die but resistant ones survive and multiply — this is selection, not directed mutation.

Question 4 · GCSE Paper 1 (AQA)
Tobacco mosaic virus (TMV) infects:
  1. Humans, causing lung disease
  2. Plants, causing mosaic patterning on leaves
  3. Bacteria, causing bacterial death
  4. Insects, causing wing deformity
Show the answer

Plants, causing mosaic patterning on leaves

Tobacco mosaic virus (TMV) infects plants, including tobacco, tomatoes, and peppers. It causes a mosaic pattern of discolouration (light and dark patches) on leaves, which reduces the plant's ability to photosynthesise. TMV was the first virus ever discovered (Dmitri Ivanovsky, 1892).

Question 5 · GCSE Paper 1 (AQA)
Which statement about osmosis in plant cells is CORRECT?
  1. A turgid cell has lost water and become floppy
  2. A plasmolysed cell has gained water and is firm
  3. A turgid cell has gained water and the vacuole pushes against the cell wall
  4. Osmosis in plants requires energy from respiration
Show the answer

A turgid cell has gained water and the vacuole pushes against the cell wall

Turgid: cell has absorbed water by osmosis → vacuole expands → pushes cytoplasm against cell wall → cell is firm and supports the plant. Flaccid: cell has lost water → vacuole shrinks → cytoplasm pulls away from cell wall slightly. Plasmolysed: severe water loss → cell membrane fully detaches from cell wall. Osmosis is passive (no energy).

Question 6 · GCSE Paper 2 (AQA)
How does insulin lower blood glucose levels?
  1. By causing the liver to release glucose into the blood
  2. By stimulating cells to absorb glucose and convert it to glycogen in the liver
  3. By reducing food intake
  4. By increasing the rate of glucagon secretion
Show the answer

By stimulating cells to absorb glucose and convert it to glycogen in the liver

Insulin lowers blood glucose by: (1) stimulating liver and muscle cells to take up glucose from the blood; (2) promoting conversion of glucose to glycogen (glycogenesis) in the liver and muscles; (3) promoting glucose uptake into all body cells for respiration. When blood glucose falls back to normal, insulin secretion decreases (negative feedback).

Question 7 · Ecology
A belt transect is used to record which species are found at regular intervals across a habitat. What change in the environment is a transect most useful for investigating?
  1. The total number of a single species
  2. Gradual changes in community linked to an environmental gradient
  3. The age structure of a population
  4. Genetic diversity within a species
Show the answer

Gradual changes in community linked to an environmental gradient

Transects are ideal for investigating zonation - how communities change along an environmental gradient such as a rocky shore (wave exposure), a woodland edge (light), or a hillside (altitude). Quadrats placed at intervals sample the community.

Question 8
In an osmosis investigation using potato chips placed in a range of sucrose solutions, percentage change in mass is calculated instead of using the raw mass change. This is because percentage change:
  1. Removes the need for a control solution
  2. Allows fair comparison between chips that had different starting masses
  3. Is required because mass cannot be measured accurately
  4. Removes the need to repeat the experiment
Show the answer

Allows fair comparison between chips that had different starting masses

Individual potato chips vary slightly in starting mass, so converting to percentage change puts every chip's result on the same scale for fair comparison.

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Common questions

How should I revise Biology for GCSE?

Short, frequent sessions of active recall beat long sessions of re-reading. Test yourself, check, and revisit anything you got wrong a day or two later rather than immediately.

Which exam board are these Biology questions for?

AQA. Content overlaps heavily between boards, so they are still useful on another spec, but check your own specification for anything board-specific.

When are the GCSE exams?

GCSEs are sat across May and June, so for the current Year 11 that means May/June 2027. Exact paper dates come from the exam boards and your school.

How many questions are there in the app?

2,406 in Biology alone, and 55,718 across 28 subjects. Signing up is free.

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